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Article: C-Terminally Truncated Forms of Tau, But Not Full-Length Tau or Its C-Terminal Fragments, Are Released from Neurons Independently of Cell Death.

Full Text (publisher's website) ; Article Metadata ; Article Data (extracted)
Kanmert D; Cantlon A; Muratore CR; Jin M; O'Malley TT; Lee G; Young-Pearse TL; Selkoe DJ; Walsh DM
J. Neurosci., 2015


Table 1.

Antibodies used, their source, and working concentrations

Antigen Antibody Source Concentration
Alix 3A9 Cell Signaling Technology 1 μg/ml
Flotillin-1 18/Flotillin-1 BD Biosciences 1 μg/ml
HIV glycoprotein 120 46-4 ATCC 1 μg/ml
PrP ICSM35 D-Gen 0.5 μg/ml
β3-tubulin ab68193 Abcam 1:250 dilution
β3-tubulin TUJ1 Covance 5 μg/ml
Tau (aa 6-18) Tau12 (human) EMD Millipore 0.1 μg/mla
Tau (aa 7-13) HJ9.4 (rodent) Gift from David Holtzman 1 μg/mla
Tau (aa 194-198) BT2 (human + rodent) Thermo Scientific 1 μg/mla,b
Tau ( aa 210-241) Tau5 (human + rodent) Cell Signaling Technology 1 μg/mlc
Tau (aa 243-441) K9JA (human + rodent) DAKO 1 μg/mla,b,c
Tau (aa 404-441) Tau46 (human + rodent) Cell Signaling Technology 1 μg/mla
Misfolded tau (residues 7-9 + 313-322) MC1 Gift from Peter Davies 5 μg/mla
  • Concentrations used for Western blotting:

  • a 5 μg/ml for IP,

  • b 2.5 μg/ml for ELISA,

  • c 5 μg/ml for ELISA.


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Inferred neuron-electrophysiology data values

Neuron Type Neuron Description Ephys Prop Extracted Value Standardized Value Content Source